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KMID : 0545120080180030573
Journal of Microbiology and Biotechnology
2008 Volume.18 No. 3 p.573 ~ p.578
Hydrolysis of Isoflavone Glucosides in Soymilk Fermented with Single or Mixed Cultures of Lactobacillus paraplantarum KM, Weissella sp. 33, and Enterococcus faecium 35 Isolated from Humans
Chun Ji-Yeon

Jeong Woo-Ju
Kim Jeong-Sang
Lim Jin-Kyu
Park Cheon-Seok
Kwon Dae-Young
Choi In-Duck
Kim Jeong-Hwan
Abstract
Lactobacillus paraplantarum KM (Lp), Weissella sp. 33 (Ws), and Enterococcus faecium 35 (Ef) were used in single (Lp, Ws, Ef) or mixed cultures (Lp+Ws, Lp+Ef, Ws+Ef) for soymilk fermentation (37oC, 12 h). After 12 h, the cell numbers, pH, and TA of soymilk were 7.4¡¿108 -6.0¡¿109 CFU/ ml, 3.8-4.5, and 0.59-0.70%, respectively. Changes in the contents of glycitin and genistin in soymilk fermented with Ef were not significant (p<0.05). The contents of isoflavone glucosides in soymilk fermented with the other cultures decreased significantly with an increase of aglycone contents (p<0.05). It corresponded well with a sharp increase in ¥â- glucosidase activity during fermentation. About 92-100% of the daidzin and 98-100% of the genistin in soymilk were converted to corresponding aglycones by Lp, Ws, or Lp+Ef within 12 h.
KEYWORD
Fermentation, isoflavones, lactic acid bacteria, soymilk
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